منابع مشابه
One-by-One Sample Preparation Method for Protein Network Analysis
Proteomics is the large-scale study of an organism's complete complement of proteins, and its relevant technologies have matured over recent years. Along with the development of mass spectrometry (MS), MS-based proteomics has emerged as an invaluable tool for largescale identification and quantification of protein networks (Aebersold & Mann, 2003; Domon & Aebersold, 2006). Proteomic data is imp...
متن کاملhigh-level soluble expression and one-step purification of htlv-i p19 protein in escherichia coli by fusion expression
expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...
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ژورنال
عنوان ژورنال: Nature Cell Biology
سال: 2006
ISSN: 1465-7392,1476-4679
DOI: 10.1038/ncb0506-429